Autoregulation of JARID2 through PRC2 interaction with its antisense ncRNA

Autoregulation of JARID2 through PRC2 interaction with its antisense ncRNA

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Abstract Objective JARID2 is a member of chromatin-modifying Polycomb Repressive Complex-2 or PRC2.It plays a role in recruiting PRC2 to developmental genes and regulating its activity.JARID2 along with PRC2 is indispensable for normal development.However, it remains unclear how JARID2 expression itself is regulated.

Recently a number of non-protein-coding RNAs or ncRNAs are shown to regulate transcription.An antisense ncRNA, JARID2-AS1, is expressed from the first intron of JARID2 isoform-1 but its role in regulation of JARID2 expression has not been investigated.The objective of this study was to explore the role click here of JARID2-AS1 in regulating JARID2 and consequently PRC2.Results We found that JARID2-AS1 is localised in the nucleus and shows anti-correlated expression pattern to that of JARID2 isoform-1 mRNA.

More interestingly, data mining approach strongly indicates that JARID2-AS1 binds to PRC2.These are important observations that provide insights into transcriptional regulation of JARID2, especially because they indicate that JARID2-AS1 by interacting and probably recruiting PRC2 participates in an auto-regulatory loop that controls levels of JARID2.This holds importance in regulation of developmental and differentiation processes.However, to support this hypothesis, further in-depth click here studies are needed which can verify JARID2-AS1-PRC2 interactions.